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Current Parts

Page history last edited by Natasha Tuskovich 14 years, 6 months ago

Parts that have been started

 

Part
 Resistance Colonies Status Plasmid
 Cryopreserved

Length

Part + plasmid

 Gel
Parts necessary for minimal NAND gate system              
*P1010-Amp (pSB1A3) Amp Yes Miniprepped -20oC -80oC 675+?  Band
*P1010-Kan (pSB1K3) Kan Yes Miniprepped -20oC -80oC 675+?  Band
*P1010-Cm (pSB1C3) (1,5E) Cm Yes Miniprepped

-20oC

(C, B, F)

 -80oC 675 + 2072 Band
R0051(plambda cI) Amp Yes Broth culture     49 + 2079 NO
I0500 (pAra) Kan Yes Miniprepped -20oC -80oC 1210 + 4425 Band
R0010 (pLac) Amp Yes Miniprepped -20oC -80oC 200 + 2079 Faint band
J23066(ribokey + terminator) Amp Yes Miniprepped -20oC -80oC 336 + 2356 Band

C0051 (λcI repressor)

 Amp Yes Miniprepped -20oC -80oC 750 + 2079 Band
J23032 (ribolock) Amp Yes Miniprepped -20oC -80oC 43+? Band
R1051 (lambda cI promoter) Amp Limited growth Has been frozen -80oC, but no bands ever. -20oC -20oC   NO
J06504 (mCherry CDS) Amp Yes Miniprepped -20oC -80oC

714+

2079

 Band
B0034 (standard RBS) Amp Yes Miniprepped -20oC -80oC 12+? Band
B0015 (double transcription stop) Amp/Kan Yes Miniprepped -20oC -80oC 129+? Band
K145303 (const. promoter+ribolock+GFP+stop) Amp Yes Miniprepped  -20oC -80oC 1069+? Band
J23102 (const. promoter) Amp Yes Miniprepped -20oC -80oC 35+? Band
E0422 (RBS+ECFP+stop) Amp Yes Miniprepped -20oC -80oC 917+? Band
J23070 (SerRiboKey) Amp   Miniprepped -20oC     Band
Thermometer parts              
J04450 (Lac controlled RFP) Amp         1069+? Band
J04450 (Lac controlled RFP) Kan Yes Miniprepped -20oC -80oC   Band
K115017 (32oC temp. sensing RBS) Amp/Tet Yes Miniprepped -20oC     Band
C0012 (LacI repressor + LVA) Amp Yes Miniprepped -20oC     Band
E0240 (RBS + GFP + stop) Amp No          
J04630 (GFP + stop) Amp/Kan   Miniprepped -20oC     Band
E0840 (RBS + GFP + stop) Amp Yes Miniprepped -20oC      
K115020 (37oC temp. sensing RBS) Amp/Tet Yes Miniprepped -20oC      
Extra parts              
I13521 (Ptet RFP generator) Amp Yes Miniprepped, but needs gel run.  -20oC -80oC    

P0151 (PoPs cI lambda) (B0031+C0051+B0015)

 Amp Yes Miniprepped -20oC -80oC   Band
J45200 (Banana odor) Amp/Tet Yes Miniprepped -20oC -80oC   Band
J45120 (Wintergreen odor) Amp/Tet Yes Miniprepped -20oC -80oC   Band
K081005 (promoter + RBS) Amp Yes Miniprepped -20oC   58+?  

K098988 (temp. sense. GFP

reporter)

 Kan Yes Miniprepped -20oC   1819+?  
C0083 (Aspartase) Kan Yes Miniprepped -20oC -80oC   Band

 

*All P1010 backbone plasmids listed here

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