Miniprep: Qiagen Miniprep Protocol
2 K235000 one from each plate
1 K235001
2 K235002 one from each plate
Insertion digest of K145303 to put into Chl backbone, and upstream digest of same to combine with ribokey part.
Put the competent cells in room temp shaker at 9.30 am with OD of 0.05
Agarose Gel Electrophoresis Protocol
Gel 1 started at 12.22 pm
| Ladder |
K235000 100 uL |
K235000 200 uL |
K235001 100 uL |
K235002 100 uL |
K235002 200 uL |
K145303 |
K081005 |
|
Band
present
|
Band |
Inconsistent Band |
*Multiple Bands |
Band |
Inconsistent Band |
Band |
No band |
| Size |
1441 |
2213 |
11057?
7833
5635
3783
2871
|
1913 |
1745 |
2130 |
|
Aug 25 gel 1.tif
-Layne
*This lane showed various faint bands, a normal strength band at 7833 and a super intense band at 3783. Has it been cotransformed with multiple plasmids as Chris suggested?
You can look at my calculations here.
Assemblies: 3A Assembly Protocol
J23102+P1010Kan = K235006
K145303+P1010Kan = K235007
K145303+K235001+P1010Cm = K235008
Digested, ligated and transformed: Transformation Protocol
Gel 2
| Ladder |
|
|
|
|
|
|
|
| Size |
2308 |
No band |
4045
2959
|
No band |
2776 |
No band |
No band |
aug 25 gel 02.tif
-Layne
Hopefully someone knows what was in this gel?
This was a really blurry gel, we can't put much weight on the values from it.
You can look at my calculations here.
Comments (0)
You don't have permission to comment on this page.