We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) colonies from each successful plate of composite parts that was transformed on the 27th. We need to start nanospec-ing our plasmids once prepped so we can tell if DNA concentration is a limiting factor. Finally we need to deal with out p1010-kan. It looks like there should be colonies of our newly rehydrated backbone when we come in tomorrow if we are lucky.
Comments (2)
james jacoby said
at 8:35 pm on Aug 30, 2009
what time are you going to be in, Chris? I think there are a few issues running around right now:
1) poor gel results. We're not sure why the gels are running like they are. Prep issue?
2) P1010 Kan - not going to be ready until Wednesday unfortunately.
3) parts k235006, 7, and 8 are all a wash. different reasons, but we need to remake them entirely if we want them.
I'll be in in the morning, but have to make it a short day to get packed and things dealt with around here...
Chris Tuttle said
at 8:19 am on Aug 31, 2009
I'm coming in for 10 give or take. see you then?
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