Miniprep 4 previous parts (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp) from overnight cultures: Qiagen Miniprep Protocol
gel 4 parts: Agarose Gel Electrophoresis Protocol
visualized the gel, pasted into Ky's notebook
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nanodropped the RFP generator following the miniprep, and using TE as the elution buffer to zero the nanodrop
21 ng/ul
260/280 ratio of 1.54
260 to 230 ratio of 1.81
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R0051 - lambda cI promoter, we re-ran the transformation protocol with more of the rehydrated pklasmid
Rehydrated and Transformed the following parts:
I0050 - arabinose promoter on a Kan plate, add IPTG to broth culture to induce high copy
R0010 - lactose promoter
J23032 - ribolock RBS
J23066 - ribokey
C0051 - lambda cI with the LVA tag
(return to Lab notebook)
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