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Lab July 27 2009

Page history last edited by Natasha Tuskovich 14 years, 8 months ago

Checked plates - growth on all 4 plates

 

new DH5alpha plates look contaminated. Staph? Not sure.

 

old competent cells look OK. Should we just go back to our old cell line? It seems the issue was a plasmid dilution issue rather than a competent cells issue.

 

re-plating with the following plasmid dilutions:

 

currently have 23ng/ul, to get to 10ng/ul mix 4.35ul plasmid and 5.65 ul dH2O

 

1 ng = 1ul of previous mix in 9ul of dH2O

 

.01 ng = 1ul of previous mix in 9ul of dH2O

 

after mixing each plasmid concentration, follow the Transformation Protocol.

 

 

(back to Lab notebook)

 

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