Alright, the plan for today is to do the assemblies K19, 20 and 21.  Rehydrate the new ribokey J23070, and retransform R0051, E0240, C0012 and K115017.

7pm

- Assembled K235019, 20, 21.

- Rehydrated J23070 (new ribokey), J04630 (GFP + stop: to use as a control)

- Transformed all of the above

- Plated all of the above plus new streak of R0051 stab (I'm not sure what kind of growth to expect from that.)

I can come in tomorrow afternoon at 4.30 to start broth cultures for these.  (Reminder to autoclave epis!  They will be needed for the miniprep the next day!)  And on Thursday I can miniprep these at 8.30am.  If no one else can be here, I can run a gel at 2.30 on Thurs but it would be great if anyone else can do it that day.