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assemblies

Page history last edited by Natasha Tuskovich 14 years, 6 months ago

Our range of part numbers is:BBa_K235000 to BBa_K235999

 

Part
 Upstream
 Downstream
 Destination Plasmid Assembled Colonies Gel Plasmid Cryopreserved
 Entry in registry?
K235000 (Chl) lactose promoter+ribokey+stop R0010(Amp) lactose promoter J23066(Amp) ribokey+stop P1010-Chl Assembled Colonies Band -20oC -80oC Registered
K235001 (Chl) constituitive (const.) promoter+ribokey+stop J23102(Amp) const. promoter J23066(Amp) ribokey+stop P1010-Chl Assembled Colonies Band -20oC -80oC Registered
K235002 (Chl) const. promoter+RBS+ECFP+stop J23102(Amp) const. promoter E0422 (Amp) RBS+ECFP+stop P1010-Chl Assembled Colonies Band -20oC -80oC Registered

K235003 (Chl)

λcI repressor+stop

 C0051 (Amp) λcI repressor B0015(Amp/Kan) stop P1010-Chl Assembled Colonies Band     Registered
K235004 (Chl) lactose promoter+RBS R0010(Amp) lactose promoter B0034 (Amp) RBS P1010-Chl Assembled No       Registered
K235005 (Chl) RFP+stop J06504 (Amp) mCherry CDS B0015(Amp/Kan) stop P1010-Chl Assembled Colonies Band -20oC   Registered
K235006 (Kan) const. promoter J23102(Amp) const. promoter N/A P1010-Kan Assembled No       no
K235007 (Kan) const. promoter+ribolock+GFP+stop K145303(Amp) const. promoter+ribolock+GFP+stop N/A P1010-Kan Assembled No       no
K235008 (Chl) const. promoter+ribolock+GFP+stop+const. promoter+ribokey+stop K145303(Amp) const. promoter+ribolock+GFP+stop K235001 (Chl) const. promoter+ribokey+stop P1010-Chl Assembled No       no
K235009 (Chl) const. promoter+ribolock J23102(Amp) const. promoter J23032 (Amp) ribolock P1010-Chl Assembled Colonies       Registered
K235010 (Chl) const. promoter+ribolock+GFP+stop K145303(Amp) const. promoter+ribolock+GFP+stop N/A P1010-Chl Assembled Colonies Band -20oC   Registered
K235011 (Amp) const. promoter+ribolock+RFP+stop K235009 (Chl) const. promoter+ribolock K235005 (Chl) RFP+stop P1010-Amp No         Registered
K235012 (Amp) const. promoter+ribolock+RFP+stop+lactose promoter+ribokey+stop K235011 (Kan) const. promoter+ribolock+RFP+stop K235000 (Chl) lactose promoter+ribokey+stop P1010-Amp No         no
K235013 (Kan) const. promoter+ribolock+GFP+stop+lactose promoter+ribokey+stop K145303(Amp) const. promoter+ribolock+GFP+stop K235000(Chl) lactose promoter+ribokey+stop P1010-Kan Assembled Colonies       Registered
K235014 (Kan) const. promoter+ribolock+GFP+stop+const. promoter+ribokey+stop K145303(Amp) const. promoter+ribolock+GFP+stop K235001(Chl) const. promoter+ribokey+stop P1010-Kan Assembled Colonies       Registered
K235015 (Chl) plambda+RBS R0051(Amp) plambda cI B0034 (Amp) RBS P1010-Chl No         Registered
K235016 (Chl) pAra+ribolock I0500(Kan) pAra J23032 (Amp) ribolock P1010-Chl Assembled Colonies       Registered
K235017 (Chl) pAra+RBS I0500(Kan) pAra B0034 (Amp) RBS P1010-Chl Assembled Colonies       Registered
K235018 (Amp) lambda promoter+RBS+RFP+stop K235015 (Chl) lambda promoter+RBS K235005 (Chl) RFP+stop P1010-Amp No         Registered
K235019 (Amp) pAra+ribolock+λcI repressor+stop K235016 (Chl) pAra+ribolock K235003 (Chl) λcI repressor+stop P1010-Amp Assembled Colonies Band -20oC   Registered
K235020 (Amp) pAra+RBS+λcI repressor+stop K235017 (Chl) pAra+RBS K235003 (Chl) λcI repressor+stop P1010-Amp Assembled Colonies Band -20oC   Registered
K235021 (Amp) const. promoter+ribolock+λcI repressor+stop K235009 (Chl) const. promoter+ribolock K235003 (Chl) λcI repressor+stop P1010-Amp Assembled Colonies Band -20oC   Registered
K235022 (Chl) lambda promoter+RBS+RFP+stop+pAra+ribolock+λcI repressor+stop K235018(Amp) lambda promoter+RBS+RFP+stop K235019 (Amp) pAra+ribolock+λcI repressor+stop P1010-Chl No         Registered
K235023 (Chl) lambda promoter+RBS+RFP+stop+ pAra+RBS+λcI repressor+stop K235018(Amp) lambda promoter+RBS+RFP+stop K235020 (Amp) pAra+RBS+λcI repressor+stop P1010-Chl No         Registered
K235024 (Amp) lambda promoter+RBS+RFP+stop+const. promoter+ribolock+λcI repressor+stop K235018(Amp) lambda promoter+RBS+RFP+stop K235021 (Amp) const. promoter+ribolock+λcI repressor+stop P1010-Chl No         Registered
K235025 (Amp) lambda promoter+RBS+RFP+stop+pAra+ribolock+λcI repressor+stop+lactose promoter+ribokey+stop K235022 (Chl) lambda promoter+RBS+RFP+stop+pAra+ribolock+λcI repressor+stop K235000 (Chl) lactose promoter+ribokey+stop P1010-Amp No         Registered
K235026 (Amp) lambda promoter+RBS+RFP+stop+pAra+ribolock+λcI repressor+stop+const. promoter+ribokey+stop K235022 (Chl) lambda promoter+RBS+RFP+stop+pAra+ribolock+λcI repressor+stop K235001 (Chl) const. promoter+ribokey+stop P1010-Amp No         Registered
K235027 (Amp) lambda promoter+RBS+RFP+stop+const. promoter+ribolock+λcI repressor+stop+lactose promoter+ribokey+stop K235024 (Chl) lambda promoter+RBS+RFP+stop+const. promoter+ribolock+λcI repressor+stop K235000 (Chl) lactose promoter+ribokey+stop P1010-Amp No         Registered
K235028 (Amp) lambda promoter+RBS+RFP+stop+const. promoter+ribolock+λcI repressor+stop+const. promoter+ribokey+stop K235024 (Chl) lambda promoter+RBS+RFP+stop+const. promoter+ribolock+λcI repressor+stop K235001 (Chl) const. promoter+ribokey+stop P1010-Amp No         Registered
   Left for extra NAND part                
K235030 (Kan) const. promoter+32oC temp. sensing RBS J23102(Amp) const. promoter K115017(Amp/Tet) 32oC temp. sensing RBS P1010-Kan Assembled Colonies Band -20oC    
K235030 (Chl) const. promoter+32oC temp. sensing RBS J23102(Amp) const. promoter K115017(Amp/Tet) 32oC temp. sensing RBS P1010-Chl Assembled Colonies Band -20oC    
K235031 (Chl) LacI repressor+LVA+stop C0012(Amp) LacI repressor+LVA B0015(Amp/Kan) stop P1010-Chl Assembled Colonies Band -20oC    
K235032 (Kan) lactose promoter+RBS+GFP+stop R0010(Amp) lactose promoter E0840(Amp) RBS+GFP+stop P1010-Kan Assembled Colonies Band -20oC    
K235033 (Kan) const. promoter+32oC temp. sensing RBS K235030(Chl) const. promoter+32oC temp. sensing RBS K235031(Chl) LacI repressor+LVA+stop P1010-Kan Assembled Colonies Band -20oC    
K235034 (Chl) const. promoter+32oC temp. sensing RBS K235033(Kan) const. promoter+32oC temp. sensing RBS J04450(Kan) Lac controlled RFP P1010-Chl

No

          
K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop K235033(Kan) const. promoter+32oC temp. sensing RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl No          
K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop K235030(Chl) const. promoter+32oC temp. sensing RBS K235005(Chl) RFP+stop P1010-Kan Assembled Colonies Band -20oC    
K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop K235030(Kan) const. promoter+32oC temp. sensing RBS J04630(Amp/Kan) GFP+stop P1010-Chl Assembled Colonies Band -20oC    
K235038 (Chl) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Chl            
K235038 (Kan) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Kan            
K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop K235038 (Chl) const. promoter+RBS K235031 (Chl) LacI repressor+LVA+stop P1010-Kan            
K235040 (Chl) const. promoter+RBS+Lac controlled RFP K235039 (Kan) const. promoter+RBS J04450(Kan) Lac controlled RFP P1010-Chl            
K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop K235039 (Kan) const. promoter+RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl            
K235042 (Kan) const. promoter+RBS+RFP+stop K235038 (Chl) const. promoter+RBS K235005(Chl) RFP+stop P1010-Kan            
K235043 (Chl) const. promoter+RBS+GFP+stop K235038 (Kan) const. promoter+RBS J04630(Amp/Kan) GFP+stop P1010-Chl            

 

 

*Sept 2. - K235010 was found to be a repeat of K235005 and was fixed.

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